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ProgutTM info letter 5/2008 The effect of Progut® on the intestinal microbiota of horses. Agrifood Research Finland 2007. 1. Introduction Well-balanced and stable intestinal microbiota has shown to have a big impact on the health and wellbeing of horses. Different factors like stress, change of feeding, illnesses and the use of antibiotics can cause intestinal disturbances which will impair the condition and performance of horses and may be very detrimental, especially with young foals. Changes in the composition of caecal and faecal microbiota have been connected for example with colitis (Reeves et al. 1996), hoof fever (Rowe et al. 1994) and lowered performance (Roneus 1993). 2. Aim The aim of the study was to investigate the effect of hydrolysed brewery yeast (Progut®) on intestinal microbiota, volatile fatty acid production and blood parameters of horses. 3. Material and methods The study was conducted by MTT Agrifood Research Finland in 2007. The bacterial composition of the faecal samples was analysed by University of Kuopio. There were 10 Finnhorse mares in the trial, 6 to 12 years by age. The horses were fed with a basal diet for three weeks before the first faecal samples for microbial analysis were taken. The concentrate-roughage ratio was 30:70 and the basal diet consisted of hay, oats, mineral feed and salt. The horses were fed three times per day. Progut® (15 g/day) was included in the diet after the first faecal samples were taken and after another three week feeding period the second faecal samples were taken. Aerobic and anaerobic total bacteria, enterobacteria, lactobacilli, lactate producing bacteria, bifidobacteria and Bacteroides-Prevotella group bacteria were determined with culturing method. The content of volatile fatty acids and pH of the faeces were also measured. Blood samples from each horse were taken two times, after the both trial periods. Statistical analysis were performed using the MIXEDprocedure of SAS. 4. Results Due to an accident one horse was taken out from the trial. The volume of the red blood cells increased (p < 0.05) and the content of fibrinogen decreased (p < 0.05) by Progut® addition. The content of iron and calcium in the blood tended to increase during the Progut feeding period. Otherwise there were no statistically significant changes in the blood parameters. The pH of the faeces varied between the horses but the average pH value remained the same during the trial periods. The dry matter content of the faeces was higher in the Progut® feeding period although the difference wasn't statistically significant (table 1). The content of lactic acid in faeces
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